Age and Ageing, Vol 28, 393-397, Copyright © 1999 by British Geriatrics Society
C Bolognesi, C Lando, A Forni, E Landini, R Scarpato, L Migliore and S Bonassi
BACKGROUND: Instability in the organization and expression of the genetic
material has been hypothesized as the basic mechanism of ageing. OBJECTIVE:
To quantify the effect of ageing on chromosomal damage as measured by
spontaneous micronuclei (MN) frequency in peripheral blood lymphocytes.
METHOD: Analysis of a large population sample from two laboratories
applying the cytokinesis-block technique and a third using traditional
interphase analysis. The age-related effect on baseline level of
micronuclei frequency and on cell proliferation measures was further
investigated in a study of peripheral blood samples from healthy subjects.
RESULTS: There was an increase of MN frequency with age. The regression
lines showed a positive slope and were statistically significant (P<
0.01) with a steeper trend for cytochalasin B-treated samples. An inverse
correlation with age was detected for the percentage of binucleated cells
in laboratories using cytochalasin B. This study confirms the increase of
basal level of MN with age. A decrease by age in proliferation efficiency
measured by the percentage of binucleated cells suggests an interference of
age-related factors on cell division. CONCLUSION: There is an increase in
MN frequency with increasing age.
ARTICLES
Chromosomal damage and ageing: effect on micronuclei frequency in peripheral blood lymphocytes
Toxicological Evaluation Unit, Genova, Italy.
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